Musciomol and flunitrazepam binding sites in a subcellular fraction enriched in rat cerebellar glomeruli |
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Authors: | F Viennot B Foucaud G Gombos |
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Institution: | (1) Centre de Neurochimie du CNRS, 5 Rue Blaise Pascal, 67084 Strasbourg Cedex, France |
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Abstract: | In the internal granular layer of the cerebellar cortex the polysynaptic complexes called glomeruli consist mainly of homogeneous populations of glutamatergic and GABAergic synapses, both located on granule cell dendrites. A subcellular fraction enriched in glomeruli was prepared from rat cerebellum, and the distribution of GABAA and of benzodiazepine binding sites between membranes derived from this fraction (fraction G) and from a total cerebellar homogenate (fraction T) was studied. The benzodiazepine and GABA binding sites were measured by the binding of agonists 3H]flunitrazepam and 3H]muscimol, respectively. The results indicate that both binding sites are present, but only slightly enriched, in the glomerular synapses. We found a muscimol/flunitrazepam binding site ratio of two, which is consistent with the enrichement of muscimol binding sites in the granular layer shown by both autoradiographic with radioactive glutamatergic ligands and in situ hybridization experiments respectively. |
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Keywords: | Cerebellar glomeruli GABAA receptors muscimol flunitrazepam |
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