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Mitochondrial DNA polymorphism in male-sterile cytoplasm of rice
Authors:K Kadowaki  T Osumi  H Nemoto  K Harada  C Shinjyo
Institution:(1) National Institute of Agrobiological Resources, Tsukuba Science City, 305 Yatabe, Japan;(2) National Agriculture Research Center, Tsukuba Science City, 305 Yatabe, Japan;(3) College of Agriculture, University of the Ryukyus, 903-01 Okinawa, Japan
Abstract:Summary Mitochondrial DNAs (mtDNAs) were isolated and purified from ten strains of rice plants with male-sterile cytoplasm. The mtDNAs were digested with the restriction endonuclease PstI and the fragment patterns produced were analysed by 0.7% agarose gel electrophoresis. Restriction fragment length polymorphism was observed among the mtDNAs analysed; there were seven different patterns for the ten examined. Our results indicate that there are a variety of mtDNAs in cytoplasmically male-sterile rice.
Keywords:Male-sterile cytoplasm  Mitochondrial DNA  Restriction fragment length polymorphism  Rice
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