Characterization of quillaja bark extracts and evaluation of their purity using liquid chromatography–high resolution mass spectrometry |
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Institution: | 1. Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, College of Pharmacy, Jinan University, Guangzhou 510632, People''s Republic of China;2. Public research platform, College of Pharmacy, Jinan University, Guangzhou 510632, People''s Republic of China;3. Key Laboratory of Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, People''s Republic of China |
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Abstract: | In the course of development of semi-preparative liquid chromatographic methods for the isolation of individual quillaja saponins from Quillaja saponaria (L.), some commercially available quillaja bark extracts revealed a distinctive and characteristic pattern of additional peaks in the chromatogram that could not be attributed to saponins commonly present in quillaja. To identify these peaks, analytical procedures based on HPLC coupled with high resolution MS detection were optimized which allowed the identification of the additional saponins Mi saponin A, Mi saponin B, Mi saponin C, madhucoside A and madhucoside B. These compounds are known to be the main saponins of the Indian plant Madhuca longifolia (L.). Tandem MS experiments were performed for the unambiguous assignment of the sapogenin. Madhuca saponins yielded a characteristic fragment of protobassic acid, whereas quillaja saponins showed a fragment of quillaic acid as expected. In addition, samples from madhuca seed kernels were analysed to verify the origin of the characteristic chromatographic peak pattern observed frequently in commercially available quillaja bark extracts. |
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Keywords: | Saponin Quillaja bark extract Mahua seed cake HPLC MS |
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