Production of functionally active <Emphasis Type="Italic">Penicillium chrysogenum</Emphasis> isopenicillin N synthase in the yeast <Emphasis Type="Italic">Hansenula polymorpha</Emphasis> |
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| Authors: | Loknath Gidijala Roel AL Bovenberg Paul Klaassen Ida J van der Klei Marten Veenhuis Jan AKW Kiel |
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| Institution: | (1) Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands;(2) DSM Anti-Infectives, DSM Gist (624-0270), P.O. Box 425, 2600 AK Delft, The Netherlands;(3) Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628, BC, Delft, The Netherlands |
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| Abstract: | Background β-Lactams like penicillin and cephalosporin are among the oldest known antibiotics used against bacterial infections. Industrially,
penicillin is produced by the filamentous fungus Penicillium chrysogenum. Our goal is to introduce the entire penicillin biosynthesis pathway into the methylotrophic yeast Hansenula polymorpha. Yeast species have the advantage of being versatile, easy to handle and cultivate, and possess superior fermentation properties
relative to filamentous fungi. One of the fundamental challenges is to produce functionally active enzyme in H. polymorpha. |
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