Synchronization of cell division ofChlorella pyrenoidosa |
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| Authors: | Dagmar Vraná |
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| Institution: | 1. Department of Technical Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 6
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| Abstract: | A modification of the synchronization method forChlorella pyrenoidosa was suggested, based on the mechanical separation of small and large cells by differential centrifugation and dilution of the population after division to a constant density (maximally 3×106 cells/ml.). Using this system, with a 16-hour day and eight hours' darkness, the ontogenetic cycle ofChlorella pyrenoidosa takes 24 hours and synchronous growth and development can be maintained for eight generations. |
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