Radical scavenging capacity and cytoprotective effect of enzymatic digests of <Emphasis Type="Italic">Ishige okamurae</Emphasis> |
| |
Authors: | Soo-Jin Heo You-Jin Jeon |
| |
Institution: | (1) Faculty of Applied Marine Science, Cheju National University, Jeju, 690 - 756, South Korea;(2) Marine and Environmental Research Institute, Cheju National University, Jeju, 695-814, South Korea |
| |
Abstract: | In order to obtain natural antioxidants of good quality from a marine bioresource, we selected the brown marine alga, Ishige okamurae, which is an abundant and unutilized source of biomass and grows near Jeju Island, South Korea. An enzymatic extraction technique
was used in order to determine the antioxidant effects of I. okamurae using commercially available food-grade digestive enzymes. In the evaluation of the radical scavenging capacity of the enzymatic
extracts from I. okamurae using an electron spin resonance (ESR) spectrophotometer, all of the enzymatic extracts showed profound dose-dependent radical
scavenging abilities. The majority of enzymatic extracts generated by proteases (except for Neutrase) had stronger hydrogen
peroxide scavenging effects than the carbohydrase extract. In particular, the cytoprotective effects of the Kojizyme extract
against H2O2 - induced DNA damage increased significantly with increasing extract concentrations in our comet assay tests. These results
showed that I. okamurae may prove to be a valuable natural source of antioxidants. |
| |
Keywords: | Ishige okamurae Brown alga Enzymatic extract Radical scavenging Cytoprotective |
本文献已被 SpringerLink 等数据库收录! |
|