Specific binding of nucleotides and NAD+ to Clostridium difficile toxin A. |
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| Authors: | M D Lobban S P Borriello |
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| Affiliation: | Department of Biochemistry, University of Glasgow, UK. |
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| Abstract: | Binding of nucleotides, a tetrapolyphosphate, and NAD+ to purified toxin A of Clostridium difficile was determined by monitoring changes in intrinsic fluorescence following excitation at 280 nm, and recording emissions at 340 nm. Binding was specific for concentrations over the range 5 to 100 microM for ATP, GTP, and their respective non-hydrolysable analogues AMP-PNP and Gpp(NH)p, tetrapolyphosphate and NAD+. |
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