Expression of an HLA-Bw6-related specificity by the HLA-Cw3 molecule |
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Authors: | Dr Corine Layet Terry Delovitch Pierre Ferrier Danielle H Caillol Bertrand R Jordan François A Lemonnier |
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Institution: | (1) Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille Cédex 9, France;(2) Charles H. Best Institute Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6 Toronto, Ontario, Canada |
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Abstract: | Radioimmunoassay of HLA-transformed mouse L cells expressing A3, A24, B7, or Cw3 HLA class I molecules with a set of monomorphic monoclonal antibodies distinguishes between A3–A24 and B7-Cw3 patterns of reactivity. Analyses with Bw6-specific monoclonal antibodies and a human alloantiserum demonstrate the expression by the HLA-Cw3 molecules of a Bw6 public specificity related to but not identical with that expressed by the HLA-B7 molecules. Exon-shuffling experiments and inhibition studies of monoclonal antibody cell-surface fixation indicate that similar parts of B7 and Cw3 molecules account for their serological cross-reactivity.Abbreviations used in this paper
2-m
beta-2 microglobulin
- EBV
Epstein-Barr virus
- mAb
monoclonal antibody
- PBS-BSA
phosphate-buffered saline supplemented with 0.2% bovine serum albumin
- PBL
peripheral blood lymphocyte
- RIA
radioimmunoassay |
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