| 腐蹄病致病因子节瘤拟杆菌蛋白酶在大肠杆菌... |
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| 引用本文： | 官云浩 Moses,E.腐蹄病致病因子节瘤拟杆菌蛋白酶在大肠杆菌...[J].微生物学报,1991,31(6):433-437. |
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| 作者姓名： | 官云浩 Moses E |
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| 摘 要： |
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| 关 键 词： | 节瘤拟杆菌 蛋白酶 表达 腐蹄病 |
Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter] |
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| Y Gong,E Moses.Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter][J].Acta Microbiologica Sinica,1991,31(6):433-437. |
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| Authors: | Y Gong E Moses |
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| Institution: | National Animal Quarantine Institute, Qingdao. |
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| Abstract: | Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay. |
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