Molecular probes for the detection of Kluyveromyces marxianus chromosomal DNA in electrophoretic karyotypes of intergeneric protoplast fusion products |
| |
| Authors: | V. Witte B. Grossmann C. C. Emeis |
| |
| Affiliation: | (1) Institut für Mikrobiologie/Biologie IV, RWTH Aachen, D-5100 Aachen, Germany;(2) Present address: Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-7800 Freiburg, Germany |
| |
| Abstract: | Random genomic DNA fragments from Kluyveromyces marxianus were cloned in order to identify chromosomal bands in pulsed field electrophoresis patterns of intergeneric hybrid strains which were obtained by protoplast fusion. Molecular hybridization data indicated that the K. marxianus parental strain might be triploid, and it showed strong chromosome length polymorphism. We analyzed the karyotype of two Saccharomyces cerevisiae/K. marxianus hybrid strains (St. 1, St. 46) with our DNA probes and with a Ty1 specific probe. We found indications for recombinational events which lead to the formation of hybrid chromosomal DNA molecules. |
| |
| Keywords: | Electrophoretic karyotyping Chromosome length polymorphism Intergeneric protoplast fusion Recombination
Kluyveromyces marxianus |
| 本文献已被 SpringerLink 等数据库收录！ |
