Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis |
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Authors: | Loida?V?Ponce José?Corado Nilka?L?Díaz Email author" target="_blank">Felix?J?TapiaEmail author |
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Institution: | (1) Laboratorio de Biologa Molecular, Instituto de Biomedicina, Universidad de Central Venezuela, Apartado 4043, Caracas, 1010A, Venezuela;(2) Departamento de Ciencias Fisiolgicas, Universidad de Carabobo, Valencia, Venezuela |
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Abstract: | We evaluated the adoptive transfer of DCs on Leishmania (L.) mexicana-infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c
mice infected during adulthood with L. (L) mexicana; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mice. A neonatal
model of infection, generated after inoculation with 5 × 105 promastigotes of L. (L) mexicana, was used as the infection control group. Sixteen hours after intraperitoneal transfer of DCs (1 × 103, 1 × 105, or 1 × 106 cells/ml), neonatal recipient BALB/c mice were infected. The adoptive transfer of DCs diminished disease progression in neonatal
mice. This reduction depends on the quantity and provenance of transferred DCs, since the effect was more evident with high
numbers of DCs from adult mice infected during adulthood and healthy neonatal mice. Protection was significantly reduced in
animals receiving DCs from healthy adult mice but it was absent in mice receiving DCs from adult mice infected during neonatal
life. These results suggest that genetic susceptibility to Leishmania infection can be modified during neonatal life, and that the period of life when antigens are encountered is crucial in influencing
the capacity of DCs to induce resistance or tolerance. |
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