Strategies to enhance cell growth and achieve high‐level oil production of a Chlorella vulgaris isolate |
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| Authors: | Chun‐Yen Chen Kuei‐Ling Yeh Huei‐Meei Su Yung‐Chung Lo Wen‐Ming Chen Jo‐Shu Chang |
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| Affiliation: | 1. Dept. of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;2. Sustainable Environment Research Center, National Cheng Kung University, Tainan, Taiwan;3. Tungkang Marine Laboratory, Taiwan Fisheries Research Institute, Pingtung, Taiwan;4. Dept. of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan |
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| Abstract: | ![]()
The autotrophic growth of an oil‐rich indigenous microalgal isolate, identified as Chlorella vulgaris C? C, was promoted by using engineering strategies to obtain the microalgal oil for biodiesel synthesis. Illumination with a light/dark cycle of 14/10 (i.e., 14 h light‐on and 10 h light‐off) resulted in a high overall oil production rate (voil) of 9.78 mg/L/day and a high electricity conversion efficiency (Ec) of 23.7 mg cell/kw h. When using a NaHCO3 concentration of 1,500 mg/L as carbon source, the voil and Ec were maximal at 100 mg/L/day and 128 mg/kw h, respectively. A Monod type model was used to describe the microalgal growth kinetics with an estimated maximum specific growth rate (μmax) of 0.605 day?1 and a half saturation coefficient (Ks) of 124.9 mg/L. An optimal nitrogen source (KNO3) concentration of 625 mg/L could further enhance the microalgal biomass and oil production, leading to a nearly 6.19 fold increase in voil value. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010 |
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| Keywords: | Chlorella vulgaris growth kinetics microalgae oil/lipid production photobioreactor |
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