Production and characterization of poly‐3‐hydroxybutyrate from biodiesel‐glycerol by Burkholderia cepacia ATCC 17759 |
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| Authors: | Chengjun Zhu Christopher T Nomura Joseph A Perrotta Arthur J Stipanovic James P Nakas |
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| Institution: | 1. Dept. of Environmental and Forest Biology, SUNY‐College of Environmental Science and Forestry, Syracuse, NY 13210;2. Dept. of Chemistry, SUNY‐College of Environmental Science and Forestry, Syracuse, NY 13210 |
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| Abstract: | Glycerol, a byproduct of the biodiesel industry, can be used by bacteria as an inexpensive carbon source for the production of value‐added biodegradable polyhydroxyalkanoates (PHAs). Burkholderia cepacia ATCC 17759 synthesized poly‐3‐hydroxybutyrate (PHB) from glycerol concentrations ranging from 3% to 9% (v/v). Increasing the glycerol concentration results in a gradual reduction of biomass, PHA yield, and molecular mass (Mn and Mw) of PHB. The molecular mass of PHB produced utilizing xylose as a carbon source is also decreased by the addition of glycerol as a secondary carbon source dependent on the time and concentration of the addition. 1H‐NMR revealed that molecular masses decreased due to the esterification of glycerol with PHB resulting in chain termination (end‐capping). However, melting temperature and glass transition temperature of the end‐capped polymers showed no significant difference when compared to the xylose‐based PHB. The fermentation was successfully scaled up to 200 L for PHB production and the yield of dry biomass and PHB were 23.6 g/L and 7.4 g/L, respectively. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 |
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| Keywords: | biodiesel‐glycerol polyhydroxyalkanoates Burkholderia cepacia polyhydroxybutyrate end‐capped PHB |
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