Salt-imposed restrictions on the uptake of macroelements by roots of Arabidopsis thaliana |
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Authors: | Houneida Attia Najoua Karray Mokded Rabhi Mokhtar Lachaâl |
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Institution: | (1) Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis El Manar, Tunisia;(2) Laboratoire d’Adaptation des Plantes aux Stress Abiotiques, Centre de Biotechnologie de Borj-Cedria, Ecopark of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia |
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Abstract: | The aim of this investigation was to identify the growth limiting factors in Arabidopsis thaliana subjected to a mild salt stress. Two natural accessions (Col and N1438) were compared. In spite of their morphological and developmental similarity, they have been previously shown to differ in
the response of their superoxide dismutase genes to salt stress (Physiol Plant 132:293–305, 2008). Thirty-day-old seedlings
were grown for 15 days using a split-root configuration in which the root system was divided into two equal parts: the first
was immersed in a complete nutrient solution with 50 mM NaCl added, while the second part was immersed in either complete
or incomplete K-, Ca-, or N-free medium. Using this approach, we demonstrated that K+ and Ca2+ uptake was impaired in the roots subjected to NaCl. There was no indication of the salt-induced inhibition of N uptake. If
K+ or Ca2+ were available from salt-free medium, plants were able to grow at normal rate and accumulate large amounts of Na+ in the shoots. These results indicate that the sensitivity of Arabidopsis growth to mild salinity was probably due to an
inhibition of K+ or Ca2+ root transport by salt rather than due to salt accumulation in shoots. Furthermore, the salt sensitivity of ion transport
in roots seemed to depend on the genotype, since K+ was limiting for Col growth, in contrast to N1438, the growth of which was limited by Ca2+. |
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Keywords: | Growth-limiting minerals Macroelements Salinity Split-root system |
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