短额负蝗精子发生过程中SBA受体的动态分布

用常规组织学方法观察短额负蝗Atractomorpha sinensis Bolivar精子发生过程中生精细胞的显微结构,并以大豆凝集素(soybean agglutinin,SBA)为探针利用凝集素细胞化学方法研究该过程中N-乙酰半乳糖复合物的分布变化。结果表明,短额负蝗精子发生经历了精原细胞增殖期、初级精母细胞期、次级精母细胞期、精子细胞形成期和精子成熟期5个时期,在这5个时期中各期生精细胞的大小、形态、核染色体等变化明显。在整个精子发生过程中,N-乙酰半乳糖复合物出现于精原细胞期,并于精母细胞期发生明显的修饰和变化,精子形成期和成熟期没有N-乙酰半乳糖复合物的表达。提示,N-乙酰半乳糖复合物的修饰和变化与短额负蝗生精细胞的生长和分化密切相关。

Distribution of SBA receptors and microstructure of spermatogenic cells during spermatogenesis of Atractomorpha sinensis

The microstructure of spermatogenic cells during spermatogenesis of Atractomorpha sinensis Bolivar was observed by the microscopic methods, and distribution of the soybean agglutinin ( SBA) receptors during spermatogenesis was detected by using lectin-binding cytochemistry method. The results demonstrated that the process of spermatogenesis in A. sinensis could be divided into 5 stages, including the spermospore proliferation stage, the primary spermatocyte stage, the secondary spermatocyte stage, the spermateliosis stage and the spermiotiliosis stage. During the whole process, great changes took place in size, morphology and chromosomes of the spermatogenic cells. The results of SBA labeling showed that the GalNAc glycoconjugates first appeared at spermospore proliferation stage, and experienced obvious modification during the spermatocyte stage, but at the spermateliosis and the spermiotiliosis stage, the GalNAc glycoconjugates disappeared. These results suggested that during the spermatogenesis of A. sinensis, the quantity and location of the GalNAc glycoconjugates apparently changed, which may be responsible for the growth and differentiation of the spermatogenetic cells.