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| 利用巴斯德毕赤酵母表达外源蛋白的研究进展 | |
| 引用本文： | 余祖华,王红宁. 利用巴斯德毕赤酵母表达外源蛋白的研究进展[J]. 生物技术通讯, 2004, 15(6): 614-616 |
| 作者姓名： | 余祖华 王红宁 |
| 作者单位： | 四川农业大学,预防医学生物工程实验室,雅安,625014 |
| 基金项目： | 国家“十五”重点科技攻关子课题(2002BA514A-12) |
| 摘 要： | 随着基因工程技术的迅速发展，已有数百种外源蛋白利用巴斯德毕赤酵母表达系统获得了成功表达。本综述了该表达系统的优点、系统的构成，外源基因转化该表达系统的方式及表达特点，阐述了该系统在生产外源蛋白上的广泛应用．并重点分析了影响外源蛋白在该表达系统中表达的因素及优化策略等。 |
| 关 键 词： | 表达系统 外源蛋白 巴斯德毕赤酵母 外源基因 基因工程技术 转化 优化策略 研究进展 影响 因素 |
| 文章编号： | 1009-0002(2004)06-0614-03 |
| 修稿时间： | 2004-01-16 |
Advances of expressing heterologous protein in Pichia pastoris |
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| YU Zu-hua,WANG Hong-ningCollege of Animal Science,Sichuan Agricultural University,Ya'an ,China. Advances of expressing heterologous protein in Pichia pastoris[J]. Letters in Biotechnology, 2004, 15(6): 614-616 | |
| Authors: | YU Zu-hua WANG Hong-ningCollege of Animal Science Sichuan Agricultural University Ya'an China |
| Affiliation: | YU Zu-hua,WANG Hong-ningCollege of Animal Science,Sichuan Agricultural University,Ya'an 625014,China |
| Abstract: | Several hundred heterologous proteins have been expressed in Pichia pastoris as the rapid progress of genic engineering. In this review, the advantages and the composings of Pichia pastoris expression system and the translated methods and the expressed characteristics of the expression system were summarized. The wide applications of producing heterologous protein in the system were expatiated. Effect factors of heterologous protein,s expression in the expression system and the strategies for optimizing the expression system were also summarized stressly. |
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| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |