Image processing: a non-destructive method for measuring growth in cell and tissue culture

The use of machine vision in evaluating callus growth directly in Petri dishes is reported for carrot cell suspension growing in the presence of increasing concentrations of various herbicides. Machine vision was much more accurate and sensitive than visual counting of the cell aggregates. Image processing also gives more detailed information than does counting because of its ability to distinguish between intermediate levels of callus growth.Abbreviations 2,4-D 2,4 dichlorophenoxyacetic acid - MS Murashige and Skoog - GR Gelrite