Multi-PRINS: multiple sequential oligonucleotide primed in situ DNA synthesis reactions label specific chromosomes and produce bands |
| |
| Authors: | E. J. M. Speel Diane Lawson A. H. N. Hopman John Gosden |
| |
| Affiliation: | (1) Department of Molecular Cell Biology, University of Limburg, PO Box, NL-6200 Maastricht, MD, The Netherlands;(2) MRC Human Genetics Unit, Western General Hospital, EH4 2XU Edinburgh, Scotland, UK |
| |
| Abstract: | A fast method for identifying several chromosomes with chromosome-specific oligonucleotide primers directing an in situ labeling reaction is described. Up to three reactions distinguished by different fluorochromes (fluorescein isothiocyanate, rhodamine/Texas red, p-aminomethyl-cyclohexane carboxylic acid) can currently be performed. Prospects for increasing this up to seven colors, and for the future of the process in prenatal diagnosis are discussed. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
