Preparation,characterization, biological activity and metabolism of all-trans retinoyl fluoride |
| |
Authors: | Arun B. Barua James A. Olson |
| |
Affiliation: | Department of Biochemistry and Biophysics, Iowa State University, Ames, IA 50011, U.S.A. |
| |
Abstract: | All-trans retinoyl fluoride was prepared by treating all-trans retinoic acid with diethylaminosulfurtrifluoride. The crystalline product, which was characterized by melting point, infrared, 1H-NMR, 19F-NMR and elementary analysis, showed λmax at 382 nm in hexane (ε = 4.98·104 M?1·cm?1) and at 392 nm in methanol (ε = 4.60·104 M?1·cm?1). Its biological activity in the rat growth assay, relative to all-trans retinyl acetate, was 22% ± 10%. Upon oral administration for 5 days to vitamin A-depleted rats, retinoyl fluoride (1020 μg) was rapidly metabolized to a polar metabolite fraction and, in the intestine, to an unstable retinol-like metabolite, purpotedly 15-fluororetinol. Upon administering intraperitoneally smaller doses (47–94 μg) of [11-3H]retinoyl fluoride, which was synthesized from [11-3H] retinoic acid, radioactive retinoic acid was noted in the liver and plasma but not in the intestine. As expected, a radioactive polar fraction appeared in the intestine and liver, but radioactive retinol, retinyl ester and some common oxidation products were not detected. Of the administered radioactivity, 72% was excreted in the urine, and only 4% was found in the feces over a 7-day period. Hydrolysis of the urine gave a radioactive fraction with a polarity similar to that of retinoic acid. Retinoyl fluoride also reacts readily with glycine to yield N-retinoyl glycine. Thus, the biological activity of retinoyl fluoride can be attributed to the formation of retinoic acid, probably by way of N-retinoyl derivatives. A possible pathway for its metabolism is presented. |
| |
Keywords: | Retinoyl fluoride Retinol derivative |
本文献已被 ScienceDirect 等数据库收录! |
|