Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential |
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| Authors: | Dautu George Munyaka Biscah Carmen Gabriella Zhang Guohong Omata Yoshitaka Xuenan Xuan Igarashi Makoto |
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| Affiliation: | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. |
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| Abstract: | A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-gamma synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes. |
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| Keywords: | Toxoplasma gondii DNA immunization DNA, deoxyribonucleic acid MIC2, microneme protein-2 M2AP, MIC2 associated protein AMA1, apical membrane antigen-1 BAG1, bradyzoite antigen-1 IgG, immunoglobulin G |
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