Molecular recognition of a DNA:RNA hybrid: sub-nanomolar binding by a neomycin-methidium conjugate |
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| Authors: | Shaw Nicholas N Xi Hongjuan Arya Dev P |
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| Affiliation: | aLaboratory of Medicinal Chemistry, Department of Chemistry, Clemson University, Clemson, SC 29634, USA |
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| Abstract: | A novel neomycin–methidium conjugate was synthesized. The covalent linkage of the aminoglycoside to an intercalator, a derivative of ethidium bromide, results in a new conjugate capable of selective recognition of the DNA:RNA hybrid duplex. Spectroscopic methods: UV, CD, fluorescence, and calorimetric techniques: DSC and ITC were used to characterize the sub-nanomolar binding displayed by the conjugate for the DNA:RNA hybrid duplex, poly(dA):poly(rU). |
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| Keywords: | DNA Aminosugar Ethidium bromide Intercalator Neomycin Aminoglycoside RNA DNA:RNA hybrid Telomerase |
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