A proteomics approach to study synergistic and antagonistic interactions of the fungal–bacterial consortium Fusarium oxysporum wild‐type MSA 35 |
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Authors: | Marino Moretti Alexander Grunau Daniela Minerdi Peter Gehrig Bernd Roschitzki Leo Eberl Angelo Garibaldi Maria Lodovica Gullino Kathrin Riedel |
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Institution: | 1. Agroinnova‐Centre of Competence for the Innovation in the Agro‐Environmental Field, University of Torino, Torino, Italy;2. Department of Microbiology, Institute of Plant Biology, University of Zurich, Zurich, Switzerland;3. Functional Genomics Center, Federal Institute of Technology (ETH) and University of Zurich, Zurich, Switzerland |
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Abstract: | Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil‐borne plant pathogen, including non‐pathogenic F. oxysporum strains. In this study, F. oxysporum wild‐type (WT) MSA 35, a biocontrol multispecies consortium that consists of a fungus and numerous rhizobacteria mainly belonging to γ‐proteobacteria, was analyzed by two complementary metaproteomic approaches (2‐DE combined with MALDI‐Tof/Tof MS and 1‐D PAGE combined with LC‐ESI‐MS/MS) to identify fungal or bacterial factors potentially involved in antagonistic or synergistic interactions between the consortium members. Moreover, the proteome profiles of F. oxysporum WT MSA 35 and its cured counter‐part CU MSA 35 (WT treated with antibiotics) were compared with unravel the bacterial impact on consortium functioning. Our study presents the first proteome mapping of an antagonistic F. oxysporum strain and proposes candidate proteins that might play an important role for the biocontrol activity and the close interrelationship between the fungus and its bacterial partners. |
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Keywords: | Biological control Fungal– bacterial association Fungal proteomics Microbiology |
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