Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data |
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Authors: | A Refoufi M A Esnault |
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Institution: | (1) Université de Rennes 1, UMR 6553 Ecobio, Batiment 14, Campus de Beaulieu, F-35042 Rennes CEDEX, France;(2) Present address: Faculté des Sciences, Université M’Hamed Bougara, 1 Avenue de l’Indépendance, 35000 Boumerdès, Algerie |
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Abstract: | Thirty five bands (alleles) from six enzyme systems and fifty seven random amplified polymorphic DNA (RAPD) fragments were
selected to analyse the genetic diversity of 33 polyploid wheatgrasses (Triticeae) populations of species Thinopyrum junceiforme and Elytrigia pycnantha, and two hybrids, one pentaploid and one novel 9-ploid. Dice’s similarity coefficient, the UPGMA-derived phenograms from
RAPD, and allozymes markers showed that the clustering of wheatgrass populations was based on ploidy level. These markers
had similar levels of diversity between populations, with high genetic similarity within the same ploidy-level and within
population’s individuals. The tetraploid Th. junceiforme populations are closely related, with a large similarity distances varied from 0.8 to 1. Based on the isozyme and RAPD analyses,
diploid taxa are related to polyploids with similarity coefficients 0.4. |
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Keywords: | Additional" target="_blank">Additional Elytrigia pycnantha isoenzymes pentaploid hybrid 9-ploid hybrid RAPD Thinopyrum junceiforme |
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