| 猪骨胶原蛋白酶解物中血管紧张素转换酶抑制剂的提纯 |
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| 引用本文： | 任维栋,李耀辉,耿秀芳,王守训,郭健.猪骨胶原蛋白酶解物中血管紧张素转换酶抑制剂的提纯[J].中国生物化学与分子生物学报,1996,12(6):693-697. |
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| 作者姓名： | 任维栋 李耀辉 耿秀芳 王守训 郭健 |
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| 作者单位： | 潍坊医学院生物化学教研室，潍坊医学院化学教研室，潍坊医学院中心实验室 |
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| 摘 要： | 将猪骨胶原蛋白粗提物用胰蛋白酶水解，经阳离子交换树脂层析，ＳｅｐｈａｄｅｘＧ－２５柱凝胶过滤，以及数次反相高效液相层析，最终获得一具有抑制血管紧张素转换酶（ＡＣＥ）活性的单一峰值的多肽。其氨基酸组成为Ｉｌｅ，Ｈｉｓ，Ｓｅｒ，Ｇｌｙ，Ａｌａ，Ｐｒｏ，Ｔｙｒ，Ｌｅｕ，Ａｓｐ．以Ｈｉｐ－Ｇｌｙ－Ｇｌｙ为底物，在ｐＨ为７．１的条件下，此肽对猪肺ＡＣＥ的Ｉ＿（５０）值为２６μｍｏｌ／Ｌ。
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| 关 键 词： | 胶原蛋白 血管紧张素转换酶 抑制剂 提纯 |
| 收稿时间： | 1996-12-20 |
Isolation of Angiotensin-converting Enzyme Inhibitor from Enzymatic Hydrolysate of Hog Bone Collagen |
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| Ren Wei-Dong,Li Yao-Hui, Geng Xiu-Fang,Wang Shou-Xun, Guo Jian.Isolation of Angiotensin-converting Enzyme Inhibitor from Enzymatic Hydrolysate of Hog Bone Collagen[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(6):693-697. |
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| Authors: | Ren Wei-Dong Li Yao-Hui Geng Xiu-Fang Wang Shou-Xun Guo Jian |
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| Institution: | (Department of Biochemistry, Central Laboratory, Weifang Medical College, Weifang 261042 |
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| Abstract: | The hydrolysate of hog bone collagen obtained by trypsin was processed through a cation exchange column chromatograph, gel filtration of Sephadex G-25, and several successive reverse phase HPLC, The final pure peptide was composed of Ile, His, Ser, Gly, Ala, Pro, Tyr, Leu,Asp. I_(50) value of the peptide for angiotensin-converting enzyme of hog lung is calculated to be approximately 26 μmol/L with Hip-Gly-Gly as substrae at PH 7.1. |
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| Keywords: | Collagen Angiotensin-converting enzyme Inhibitor Purification |
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