基于荧光检测的新型细胞传感器

主要介绍了一类基于荧光检测的新型细胞传感器,这类传感器利用免疫细胞表面分子特异性识别、结合抗原的特性和生物(或化学)发光技术,通过检测荧光信号在数分钟内达到检测病原体或其他抗原的目的.这类传感器的发光原理主要是利用钙离子敏感型化学荧光探针发光,如Fluo-4等,或钙离子敏感型发光蛋白发光,如水母发光蛋白、绿色荧光蛋白等.现在已经应用的主要是B细胞传感器和肥大细胞传感器.这类传感器具有灵敏度高、检测准确、反应速度快的优点.同时又存在交叉反应、细胞不易保存等不足之处.这类传感器在疾病诊断、环境监测、生物战剂检测等领域具有较大的应用前景.

Novel Cell-based Biosensors Based on Measurement of Fluorescence

The diagnosis of infectious diseases, the monitoring of environment and detection of potential bioterrorism agents greatly require a pathogen identification method with better combined speed, accuracy and sensitivity. Here, a kind of novel cell-based biosensors based on measurement of fluorescence is introduced, which could detect pathogens and other antigens by measuring fluorescent signals within minutes. It is based on the specifically bind character of antigens and antibodies and the theory of bioluminescence or chemiluminescence. The cell could emit light via calcium chemically fluorescent indicators such as Fluo-4, or calcium fluorescent proteins such as aequorin, green fluorescent protein. B cell-based biosensors and mast cell-based biosensors have been applied in some fields. This kind of biosensors has advantages in combined sensitivity, accuracy and speed, while it also has disadvantages such as cross reactivity and problems with cellular storage and maintenance. This is a promising kind of biosensors applied in the diagnosis of infectious diseases, the monitoring of environment and detection of potential bioterrorism agents.