In vitro regeneration of plantlets from shoot callus of mature trees ofDalbergia latifolia |
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Authors: | V Ravishankar Rai K S Jagadish Chandra |
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Institution: | (1) Department of Post Graduate Studies and Research in Botany, University of Mysore, Manasagangotri, 570 006 Mysore, India |
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Abstract: | A successful procedure was established for in vitro mass multiplication of Indian rosewood (Dalbergia latifolia Roxb.). In vitro regeneration of plantlets was achieved from callus of shoot tips and shoot segments of over 50-year-old elite trees on Murashige & Skoog's medium containing naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). For rooting, regenerated shoots from the calli were excised and first treated with White's liquid medium or half-strength Murashige & Skoog's medium, supplemented with indole-3-acetic acid, indole-3-butyric acid and naphthaleneacetic acid for 48 h to 72 h. Following this treatment, plantlets were transferred to hormone-free half-strength MS medium. Rooted plantlets were then transferred to pots and grown in the greenhouse.Abbreviations BAP
6-benzylamino pruine
- CH
casein hydrolysate
- CM
coconut milk
- 2, 4-D
dichlorophenoxyacetic acid
- GA
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- NAA
naphthaleneacetic acid
- PVP-10
polyvinyl pyrrolidone
- YE
yeast extract |
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Keywords: | Dalbergia latifolia plant regeneration tissue culture mass multiplication |
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