Incorporation of Labeled Precursors into the RNA and Proteins of Lactic Dehydrogenase Virus |
| |
| Authors: | Akira Niwa Shudo Yamazaki John Bader Abner Louis Notkins |
| |
| Affiliation: | aLaboratory of Microbiology and Immunology, National Institute of Dental Research and Chemistry Branch, National Cancer Institute, Bethesda, Maryland 20014 |
| |
| Abstract: | Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with (3)H-uridine and (3)H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm(3). The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 x 10(6). |
| |
| Keywords: | |
|
|
