用于绿豆种质资源遗传多样性分析的SSR及STS引物的筛选

目前能够用于绿豆(Vigna radiate)种质资源遗传多样性分析的PCR引物极其有限。通过12份农艺性状差异较大的绿豆种质对绿豆以及小豆(Vigna angularis)、豇豆(Vigna unguiculata)、菜豆(Phaseolus vulgaris)等近缘食用豆中的PCR引物进行筛选,结果表明41对绿豆SSR引物中能够有效扩增的有35对,6对有多态性;28对绿豆STS引物中有23对能够有效扩增,2对有多态性;8对小豆SSR引物能够有效扩增的有6对,但均无多态性;27对豇豆SSR引物能够有效扩增的有17对,1对有多态性;24对菜豆SSR引物能够有效扩增的有9对,1对有多态性。这些多态性引物的获得将有助于中国绿豆种质资源的遗传多样性分析。

The Screening of SSR and STS Markers for Genetic Diversity Analysis of Mungbean Germplasm

At present,PCR markers for genetic diversity analysis of mungbean(Vigna radiate) germplasm are much limited.Twelve mungbean accessions with different agronomic traits were used to screen polymorphic PCR markers that were obtained from mungbean,adzuki bean(Vigna angularis),cowpea(Vigna unguiculata) and common bean(Phaseolus vulgaris).The results showed that 35 markers among 41 mungbean SSR primer pairs were effectively amplificated and 6 were polymorphic;22 among 28 mungbean STS primer pairs were effectively amplificated and 2 were polymorphic;6 among 8 adzuki bean SSR primer pairs were effectively amplificated,but no polymorphic was found;17 among 27 cowpea SSR primer pairs were effectively amplificated,one was polymorphic;9 primer pairs had good amplification product among 24 common bean SSR primer pairs,one of them was polymorphic.These polymorphic primers will be useful for genetic diversity analysis of Chinese mungbean germplasm.