High-frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.) |
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Authors: | Jeffrey D Griffin Margaret S Dibble |
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Institution: | (1) Department of Plant, Soil, and Entomological Sciences, University of Idaho, 83844-2339 Moscow, ID, USA |
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Abstract: | Shoot regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.) was tested on MS basal medium supplemented with four different growth regulators. Regeneration frequencies for medium supplemented with 10 M 2,4-dichlorophenoxyacetic acid (2,4-D), 60 M 4amino-3, 5,6-picolinic acid (picloram), or 30 M 3,6dichloro-o-anisic acid (dicamba) ranged from 0.4 to 4%. Medium supplemented with 30 M dicamba plus 10 M 6-benzylaminopurine (BA) resulted in regeneration of shoots from 20% of the calli tested. Higher rates of growth regulators (60 or 90 M dicamba, 20 M BA) resulted in regeneration of shoots from 45% of calli of the cultivar Baron. In a subsequent study, the response of 12 North American cultivars grown on these media was cultivar-specific, with mean frequencies of regeneration ranging from 4% to 40%.Abbreviations 2,4-D
2,4-dichlorophenoxyaceticacid
- dicamba
3,6-dichloro-o-anisic acid
- picloram
4-amino-3,5,6-picolinic acid
- BA
6-benzylaminopurine |
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Keywords: | Regeneration Poa pratensis L |
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