| Abstract: | Affinity chromatography on cross-linked starch affords a simple and rapid procedure for alpha-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the alpha-amylase which is then eluted with 2M maltose solution. alpha-amylase can be obtained in a pure form with a 60% yield. The exoenzyme beta-amylase (EC 3.2.1.2) is not retained by the support and is eluted with other contaminant proteins. Therefore, this procedure allows the separation of the endo- and exoamylase activities. |
