Multiple proteins bind to the P2 promoter region of the zein gene pMS1 of maize |
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Authors: | Uwe-G Maier Klaus D Grasser Michael M Haa? and Günter Feix |
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Institution: | (1) Institute of Biology III, Schänzlestrasse 1, D-7800 Freiburg, Germany;(2) Present address: Institute for Biology II, Cell Biology, Schänzlestrasse 1, D-7800 Freiburg, Germany |
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Abstract: | Summary A 216 by promoter fragment of the 19 kDa protein zein gene pMS1, containing the CCAAT and TATA boxes, was analysed by a variety of techniques for in vitro interactions with nuclear proteins from endosperm tissue. HMG proteins were found to form stable complexes with these A/T-rich promoter sequences and several specific DNA-binding proteins appear to be involved in the formation of DNA-protein complexes with this fragment. A 29 bp region spanning the two CCAAT boxes was protected from DNase I digestion in footprinting experiments. |
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