Voltage activation of heart inner mitochondrial membrane channels |
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Authors: | Dmitry B Zorov Kathleen W Kinnally Henry Tedeschi |
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Institution: | (1) Department of Bioenergetics, A. N. Belozersky Laboratory of Molecular Biology, Moscow State University, 119899 Moscow, USSR;(2) Department of Biological Sciences, State University of New York at Albany, 12222 Albany, New York |
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Abstract: | The patch clamp records obtained from mitoplast membranes prepared in the presence of a calcium chelator generally lack channel activity. However, multiconductance channel (MCC) activity can be induced by membrane potentials above ±60mV Kinnallyet al., Biochem. Biophys. Res. Commun.
176, 1183–1188 (1991)]. Once activated, the MCC activity persists at all voltages. The present report characterizes the activation by voltage of multiconductance channels of rat heart inner mitochondrial membranes using patch-clamping. In some membrane patches, the size of single current transitions progressively increases with time upon application of voltage. The inhibitor cyclosporin has also been found to decrease channel conductance in steps. The results suggest that voltage-induced effects which are inhibited by cyclosporin Aare likely to involve either an increase in effective pore diameter or the assembly of low-conductance units. In activated patches, we have found at high membrane potentials (e.g., 130 mV) changes in conductance as high as 5 nS occurring in large steps (up to 2.7 nS). These were generally preceded by a smaller transition. Similar results were obtained less frequently at lower voltages. These results can be explained on the assumption that once assembled the channels may act in unison. |
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Keywords: | Inner mitochondrial membrane channels voltage activation assembly cyclosporin patch-clamp permeability transition pore |
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