Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension |
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Authors: | Olsson Ingmar Larsson Kjell Palmgren Ronnie Bjellqvist Bengt |
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Institution: | Proteomics Product Development, Amersham Biosciences, Uppsala, Sweden. |
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Abstract: | Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two-dimensional (2-D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak). Use of DeStreak also results in 2-D maps with simplified spot patterns and improved reproducibility. |
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