| 感染水椰八角铁甲的绿僵菌的分子鉴定及致病力测定 |
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| 引用本文： | 徐利,蓝江林,侯有明,陈玉森,陈智雄,翁章权.感染水椰八角铁甲的绿僵菌的分子鉴定及致病力测定[J].昆虫知识,2011,48(4):922-927. |
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| 作者姓名： | 徐利 蓝江林 侯有明 陈玉森 陈智雄 翁章权 |
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| 作者单位： | 1. 农业部亚热带农业生物灾害与治理重点开放实验室,福建农林大学植物保护学院,福州,350002
2. 福建省农科院农业生物资源研究所,福州,350003 |
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| 基金项目： | 科技部"973"项目，国家自然科学基金 |
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| 摘 要： | 在室内饲养的水椰八角铁甲Octodonta nipae(Maulik)种群中,发现有大量甲虫被病原菌感染致死.对死虫体表的病原真菌进行分离鉴定,并依据ITS序列分析鉴定,确定该病原真菌为金龟子绿僵菌小孢变种(Metarhizium anisopliae var.anisopliae).经室内致病力测定,接种浓度分别为1...
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| 关 键 词： | 水椰八角铁甲 金龟子绿僵菌小孢变种 ITS序列分析 致病力测定 |
Molecular identification and pathogenicity assay on Metarhizium against Octodonta nipae ( Coleoptera: Chrysomelidae) |
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| XU Li,LAN Jiang-Lin,HOU You-Ming,CHEN Yu-Sen,CHEN Zhi-Xiong,WENG Zhang-Quan.Molecular identification and pathogenicity assay on Metarhizium against Octodonta nipae ( Coleoptera: Chrysomelidae)[J].Entomological Knowledge,2011,48(4):922-927. |
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| Authors: | XU Li LAN Jiang-Lin HOU You-Ming CHEN Yu-Sen CHEN Zhi-Xiong WENG Zhang-Quan |
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| Institution: | XU Li1 LAN Jiang-Lin2 HOU You-Ming1 CHEN Yu-Sen1 CHEN Zhi-Xiong1 WENG Zhang-Quan1 |
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| Abstract: | High adult and larval mortality due to infection with a pathogenic fungus was observed when rearing Octodonta nipae (Maulik) under laboratory conditions.ITS sequence analysis identified the fungus as Metarhizium anisopliae var.anisopliae.The effect of inoculating 3rd instar larvae and adults of O.nipae with six different concentrations of M.anisopliae var.anisopliae was investigated.Dose-response bioassays showed that M.anisopliae var.anisopliae had a high level of toxicity to both larvae and adults.At conc... |
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| Keywords: | Octodonta nipae Metarhizium anisopliae var anisopliae ITS sequence analysis virulent bioassays |
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