Agrobacterium-mediated transformation of Asparagus officinalis L. long-term embryogenic callus and regeneration of transgenic plants |
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Authors: | Bruno Delbreil Philippe Guerche M Jullien |
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Institution: | (1) Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles Cedex, France;(2) Laboratoire in vitro, Jacques Marionnet GFA, 41230 Soings en Sologne, France |
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Abstract: | Summary Twenty-three independent kanamycin resistant lines were obtained after cocultivation of longterm embryogenic cultures of three Asparagus officinalis L. genotypes with an Agrobacterium tumefaciens strain harboring ß-glucuronidase and neomycin phosphotransferase II genes. All the lines showed ß-glucuronidase activity by histological staining. DNA analysis by Southern blots of the kanamycin resistant embryogenic lines and of a plant regenerated from one of them confirmed the integration of the T-DNA.Abbreviations GUS
ß-glucuronidase
- X-Gluc
5-bromo-4-chloro-3indolyl ß-D-glucuronic acid
- NPT II
neomycin phosphotransferase II |
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Keywords: | Agrobacterium tumefaciens Asparagus offlcinalis L Somatic embryogenesis Transformation |
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