Bsr-REMI: An improved method for gene tagging using a new vector inDictyostelium |
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| Authors: | Takahiro Morio Hiroyuki Adachi Kazuo Sutoh Kaichiro Yanagisawa Yoshimasa Tanaka |
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| Institution: | (1) Institute of Biological Sciences, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(2) Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, 153 Tokyo, Japan |
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| Abstract: | Using a plasmid pBsr2 which carries a blasticidin S-resistant gene, we have improved the method of REMI (restriction enzyme-mediated
integration) provided for insertional mutagenesis inDictyostelium discoideum (bsr-REMI). To confirm usefulness of thebsr-REMI, transformation efficiency, copy number of integrated DNA, and randomness of integration into genome were examined. |
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| Keywords: | Bsr-REMI Dictyostelium discoideum Insertional mutagenesis |
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