| 内切型菊粉酶生产菌株的筛选及诱变选育 |
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| 引用本文： | 王艳,金征宇,徐学明,王伟.内切型菊粉酶生产菌株的筛选及诱变选育[J].生物技术,2001,11(1):26-28. |
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| 作者姓名： | 王艳 金征宇 徐学明 王伟 |
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| 作者单位： | 无锡轻工大学食品学院 |
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| 摘 要： | 采用透明圈法筛选得到了产菊粉酶的多株菌株，并得到了1株产内切型菊粉酶较高的隐球酵母属（Cryptococcus)菌株L1，以L1作为出发菌株经Co^60诱变后，得到1株产内切型菊粉酶最好菌株C10，其诱变后低聚果糖得率比诱变前提高了52．6％，酶活力提高了51．9％。
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| 关 键 词： | 内切型菊粉酶 CO^60诱变 低聚果糖 酶活力 生产菌株 诱变选育 |
| 文章编号： | 1004-311(2001)01-0026-03 |
| 修稿时间： | 2000年9月10日 |
Screening and mutation breeding of strains for endo- inulinase production |
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| WANG Yan,JIN Zheng-yu,XU Xue-ming,WANG Wei.Screening and mutation breeding of strains for endo- inulinase production[J].Biotechnology,2001,11(1):26-28. |
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| Authors: | WANG Yan JIN Zheng-yu XU Xue-ming WANG Wei |
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| Abstract: | The wild yeast Li, which produced endo - inulinase, was obtained by see - through circile way of screening. After treatment by Co60, a regenerated mutant C10 of high yielded endo - inulinase was ob-tained, its fructooligosaccharides concentration was 52.6% and the enzamatic activity was 51.9% higher than that of L1 respectively. |
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| Keywords: | endo-inulinase Co60mutation enzamatic activity |
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