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A method for the quantitation of indole auxins in the picogram range by high performance gas chromatography of their N-heptafluorobutyryl methyl esters
Authors:Michel Hofinger
Affiliation:Laboratory of Plant Biology, University of Liège, Quai Van Beneden, 22 B-4020 Liège, Belgium
Abstract:A method is described for the extraction and HPGC-ECD quantitation of indolic acids in the picogram range. It includes an improved GLC technique and a new derivatization reagent; it is applied to the estimation of IAA in Bryonia leaves.
Keywords:Indolic compounds  plant hormones  HPGC-ECD  derivatization
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