An experiment in enzyme evolution studies withPseudomonas aeruginosa amidase |
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Authors: | Patricia H Clarke Robert Drew |
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Institution: | (1) Department of Biochemistry, University College London, Gower Street, WC1E 6BT London, UK;(2) Present address: Glebe House, School Hill, GL7 2LS Cirencester, Gloucestershire |
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Abstract: | The regulation of amidase synthesis inP. aeruginosa is under positive control. This review describes the experimental evolution of amidase and its regulator protein for the hydrolysis of novel substrates and experiments to elucidate the mechanism of the control system. |
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Keywords: | Pseudomonas aeruginosa enzyme evolution amidase |
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