生长调节剂和渗透调节物质对水曲柳体胚发生的影响

以水曲柳成熟合子胚子叶为材料诱导体胚发生，通过改变诱导培养基中植物生长调节剂和渗透调节物质的种类及浓度，分析二者对水曲柳体胚发生的影响。研究结果表明：诱导培养基中的生长调节剂在水曲柳体胚发生过程中是必不可少的因素；高渗透压有利于体胚诱导：在添加生长调节剂的条件下，添加75 g·L^-1的蔗糖可提高体胚诱导率；通过在诱导培养基中添加100 g·L^-1蔗糖产生的体胚在含有BA的分化培养基上分化率更高。研究结果为提高水曲柳体胚诱导率、改善体胚发生状况和优化体胚发生体系奠定了基础。

Effect of Plant Growth Regulators and Osmoticums on Somatic Embryogenesis of Fraxinus mandshurica Rupr.

An experiment was conducted to study the effects of plant growth regulators (PGRs) and osmotic adjustment substances on somatic embryogenesis of Fraxinus mandshurica Rupr. by changing the kinds and concentrations of them, which was taken cotyledon of mature zygotic as explants. PGRs were the essential factors for somatic embryogenesis of F.mandshurica on the induction medium. The induction medium higher osmotic stress was beneficial to induce somatic embryos. Under PGRs, the induction medium of highest SSEs induction rate was added with 75 g·L^-1 sucrose. The somatic embryo was induced by the induction medium was added with 100 g·L^-1 sucrose had higher differentiation rate on the differential medium contained BA. This study laid the foundation in increasing SE induction rate and improving the status of somatic embryogenesis and optimizing the somatic embryogenesis system of F.mandshurica.