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Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids
Authors:Karlova Rumyana  Weemen-Hendriks Mieke  Naimov Samir  Ceron Jairo  Dukiandjiev Stefan  de Maagd Ruud A
Affiliation:Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria.
Abstract:We investigated the role of domain III of Bacillus thuringiensis delta-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.
Keywords:Bacillus thuringiensis   δ-Endotoxin   Cry1Ac   Hybrid protein   Heliothis virescens
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