The Effect of a Submersion Pretreatment on In Vitro Explant Growth and Shoot Regeneration from Hypocotyls of Flax (Linum Usitatissimum) |
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| Authors: | Yildiz Mustafa Özgen Murat |
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| Institution: | (1) Department of Field Crops, Faculty of Agriculture, Ankara University, 06110 Diskapi, Ankara, Turkey |
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| Abstract: | This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and shoot regeneration on MS (Murashige and Skoog, 1962) medium supplemented with 1 mg l−1 BAP (6-benzylaminopurine) and 0.02 mg l−1 NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to
the highest values with respect to shoot regeneration percentage, shoot number per hypocotyl, shoot length and total shoot
number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured
in vitro. |
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| Keywords: | in vitro explant growth in vitro shoot regeneration Linum usitatissimum tissue water content |
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