Ultrastructural immunolocalization of actin in a fungus |
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| Authors: | T M Bourett R J Howard |
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| Institution: | (1) Central Research and Development Department, E. I. du Pont de Nemours & Company, P.O. Box 80402, 19880-0402 Wilmington, DE, USA |
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| Abstract: | Summary We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogenMagnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognizeM. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper.Abbreviations IEM
immuno-electron microscopy
- TEM
transmission electron microscopy |
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| Keywords: | Actin Freeze substitution Fungi Hyphal tip Immunocytochemistry Magnaporthe grisea |
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