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Site-Directed Mutagenesis of Stahphylococcal Nuclease: Role of Tyrosine Residues in Substrate Binding and Catalysis |
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| Authors: | E. Uhlmann J. A. Smith |
| Affiliation: | 1. Department of Molecular Biology , Genetics, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts, 02114;2. HOECHST AG, P.O. Box 80 03 20, 6230, Frankfurt (Main), 80, West Germany;3. Department of Molecular Biology and Pathology , Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts, 02114 |
| Abstract: | Abstract Oligonucleotide directed mutagenesis was used to change specific aminoacid residues in the active site of Staphylococcal nuclease. From the determined kinetic parameters we have calculated the interaction energy of the Tyr side chain with the substrate nitropheny1-pTp. |
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