Synthesis and Properties of DNA Dumbbells Containing Chemically Active Substituted Pyrophosphate Internucleotide Groups |
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Authors: | Svetlana A Kuznetsova Marta Blumenfeld Marc Vasseur Zoe A Shabarova |
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Institution: | 1. Joint Laboratory GENSET-Laboratory of Chemistry of Nucleic Acid , Moscow State University , Moscow, 119899, Russia;2. GENSET , 1 rue R. et S. Delaunay, 75011, Paris, France |
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Abstract: | Abstract DNA dumbbells with substituted pyrophosphate groups at a definite position of the sugar-phosphate backbone were synthesized by condensation of terminal 5′-phosphomonester- and 3′-methylphosphodiester groups in nicked dumbbells. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide was used as a condensing agent. An efficient method for producing extended oligonucleotides carrying an O-methyl-substitued 3′-phosphate group was developed. Properties of the modified DNA-dumbbells were investigated. The substituted pyrophosphate group in the DNA dumbbells was efficiently cleaved under the action of N-methylimidazole or ethylendiamine aqueous solutions at pH 8.0. |
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