Enhanced Antisense Effects Resulting from an Improved Streptolysin-O Protocol for Oligodeoxynucleotide Delivery into Human Leukaemia Cells |
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| Authors: | R. V. Giles J. Grzybowski D. G. Spiller D. M. Tidd |
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| Affiliation: | Department of Biochemistry , Liverpool University , P.O. Box 147, Liverpool, Merseyside, L69 3BX, UK |
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| Abstract: | Abstract An improved protocol for delivering oligodeoxynucleotides into the cytoplasm and nucleus of human cells in culture using streptolysin O is described. The new procedure permitted reduced target protein expression to result from antisense suppression of target mRNA levels. |
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