Modifications of adenylate and guanylate cyclase activities during multiplication of KB cells.

Adenylate and guanylate cyclase activities do not vary in concert during the multiplication of KB cells. Adenylate cyclase activity is low and slightly increases at cell confluency, guanylate cyclase activity, great in sparce cells, decreases during cell multiplication period. These variations are not caused by a modification of catalytic sites because the apparent Km for ATP or GTP is not changed, but by a modification of the dependance on Mg⁺⁺ or Mn⁺⁺ ions. Fresh serum increases guanylate cyclase activity but does not affect adenylate cyclase.