Improved method for ribonuclease zymogram |
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| Authors: | J M Thomas M E Hodes |
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| Affiliation: | Department of Medical Genetics, Indiana University School of Medicine, Indianapolis, Indiana 46223 USA |
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| Abstract: | An improved method for ribonuclease zymogram in agarose overlays is described which enables significantly enhanced sensitivity and the avoidance of false-positive activity. Two critical aspects of the zymogram method—the fixation/staining technique and the RNA substrate concentration—were evaluated for the RNase activity in RNase A and in human serum and urine. The zymogram method is sensitive to at least 10 pg of RNase A and does not show a false-positive reaction from 1500 μg of non-RNase protein following electrophoresis in thin-layer polyacrylamide gel. |
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| Keywords: | To whom correspondence should be addressed present address: Department of Chemistry University of Georgia Athens Ga. 30602. |
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