| 脂质体法和电穿孔法转染哺乳动物细胞研究 |
| |
| 引用本文： | 钱锋,肖成祖.脂质体法和电穿孔法转染哺乳动物细胞研究[J].生物化学与生物物理进展,1999,26(3):289-291. |
| |
| 作者姓名： | 钱锋 肖成祖 |
| |
| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
| |
| 摘 要： | 用脂质体法和电穿孔法分别转染Cos-7，Vero和Namalwa细胞.发现脂质体法在转染效率和操作方便方面比电穿孔法优越，而电穿孔法对细胞种类的适用性方面似乎比脂质体法广. 结果表明，电穿孔法能转染Cos-7，Namalwa和Vero细胞,而用脂质体法只能转染Cos-7和Vero细胞.
|
| 关 键 词： | 转染，脂质体转染，电穿孔，比较研究 |
| 收稿时间： | 1998/2/26 0:00:00 |
| 修稿时间： | 6/4/1998 12:00:00 AM |
Comparison of Lipofection and Electroporation Gene Transfer Into Mammalian Cells |
| |
| QIAN Feng and XIAO Cheng-Zu.Comparison of Lipofection and Electroporation Gene Transfer Into Mammalian Cells[J].Progress In Biochemistry and Biophysics,1999,26(3):289-291. |
| |
| Authors: | QIAN Feng and XIAO Cheng-Zu |
| |
| Institution: | Institute of Biotechnology, Military Medicine Academy, Beijing 100071, China;Institute of Biotechnology, Military Medicine Academy, Beijing 100071, China |
| |
| Abstract: | The transfection of Cos-7, Vero and Namalwa cell lines by lipofection and electroporation methods have been examined. The lipofection method was found to be better than electroporation method in terms of higher transfection and convenient use. The electroporation method is superior to the lipofection method in terms of average transfection efficiency for a wider variety of cells lines. It was found that Namalwa cells could only be transfected by electroporation method, while Cos-7 and Vero cells could be transfected by both methods. |
| |
| Keywords: | transfection lipofection electroporation comparison study |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
