Sequence and transfection of BoLA-DRB3 cDNAs |
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Authors: | Russell G C Fraser D C Craigmile S Oliver R A Dutia B M Glass E J |
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Institution: | Division of Molecular Biology, Roslin Institute, Midlothian, UK. |
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Abstract: | Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies. |
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Keywords: | cattle MHC genes transfection phenotyping |
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